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Polydispersity of liposome preparations as a likely source of peak width in capillary zone electrophoresis
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SYSNO ASEP 0162276 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Polydispersity of liposome preparations as a likely source of peak width in capillary zone electrophoresis Author(s) Radko, S. P. (US)
Šťastná, Miroslava (UIACH-O) RID, ORCID
Chrambach, A. (US)Source Title Journal of Chromatography-B. - : Elsevier BV - ISSN 0378-4347
Roč. 761, č. 1 (2001), s. 69-75Number of pages 7 s. Language eng - English Country US - United States Keywords polydispersity ; liposomes Subject RIV CB - Analytical Chemistry, Separation CEZ AV0Z4031919 - UIACH-O Annotation Negatively charged liposomes consisting of phosphatidylcholine/phosphatidylglycerol/cholesterol in various ratios when subjected to capillary zone electrophoresis (CZE) in Tris-HCl (pH 8) buffer of different concentrations have been shown previously to exhibit a size-dependent migration rate at low ionic strength. The present study, focusing on the peak width under those conditions, shows that the polydispersity of liposomes correlated with, and appears to be a dominant source of, the peak width of the liposomes in CZE in a buffer of low ionic strength (2 to 5 mM Tris-HCl buffer, pH 8) at moderate electric field strengths (200 V cm(-1) or less). This finding, beyond allowing for the analysis of liposome polydispersity by CZE, suggests that the size-dependent fractionation of liposome preparations by a preparative electrophoretic technique such as free-flow electrophoresis is potentially feasible. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2002
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