0162275 - UIACH-O 20010021 RIV US eng J - Journal Article
Buzas, Z. - Chang, H. T. - Vieira, N. E. - Yergey, A. L. - Šťastná, Miroslava - Chrambach, A.
Direct vertical electroelution of protein from a PhastSystem band for mass spectrometric identification at the level of a few picomoles.
Proteomics. Roč. 1, č. 5 (2001), s. 691-698. ISSN 1615-9853. E-ISSN 1615-9861
Institutional research plan: CEZ:AV0Z4031919
Keywords : vertical electroelution * picomolar protein identification * mass spectrometry
Subject RIV: CB - Analytical Chemistry, Separation

An electroelution apparatus prototype of a new design was constructed. In that design, the electric field passes vertically through the protein band located on horizontal minigel polymerized on a net of Gel-Fix. An apparatus, allows for electroelution of protein bands at the level of a few picomoles and their identification, after concentration, by matrix-assisted laser desorption/ionization-time of flight mass spectrometry.
Permanent Link: http://hdl.handle.net/11104/0059594