0162274 - UIACH-O 20010019 RIV US eng J - Journal Article
Zhang, B. - Foret, František - Karger, B. L.
High throughput microfabricated CE/ESI-MS: automated sampling from a microwell plate.
Analytical Chemistry. Roč. 73, č. 11 (2001), s. 2675-2681. ISSN 0003-2700. E-ISSN 1520-6882
Institutional research plan: CEZ:AV0Z4031919
Keywords : electrospray mass-spectrometry * dead volume * electrophoresis
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 4.532, year: 2001

A new design for high-throughput microfabricated capillary electrophoresis/electrospray mass spectrometry (CE/ ESI-MS) with automated sampling from a microwell plate is presented. The approach combines a sample-loading port, a separation channel, and a liquid junction, the latter for coupling the device to the R IS with a miniaturized subatmospheric electrospray interface. The microdevice was attached to a polycarbonate manifold with external electrode reservoirs equipped for electrokinetic and pressure-fluid control. A computer-activated electropneumatic distributor was used for both sample loading from the microwell plate and washing of channels after each run. Removal of the electrodes and sample reservoirs from the microdevice structure significantly simplified the chip design and eliminated the need both for drilling access holes and for sample/buffer reservoirs. The external manifold also allowed the use of relatively large reservoirs that are necessary for extended time operation of the system. Initial results using this microfabricated system for the automated CE/ESI-MS analysis of peptides and protein digests are presented.
Permanent Link: http://hdl.handle.net/11104/0059593