0162256 - UIACH-O 20010020 RIV NL eng J - Journal Article
Janoušková, Jana - Budinská, Marcela - Plocková, Jana - Chmelík, Josef
Optimization of experimental conditions for the separation of small and large starch granules by gravitational field-flow fractionation.
Journal of Chromatography A. Roč. 914, 1-2 (2001), s. 183-187. ISSN 0021-9673. E-ISSN 1873-3778
R&D Projects: GA AV ČR IAA4031805
Grant - others:Copernicus(BE) ERB3512-PL979070
Institutional research plan: CEZ:AV0Z4031919
Keywords : field-flow fractionation * optimization * flow-rate gradients
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 2.793, year: 2001

Separation of small and large barley starch granules by gravitational field-flow fractionation (GFFF) was investigated from the point of sample pre-treatment, amount of injected sample, and elution conditions. It was concluded that it is reasonable to soak the starch granules for at least 24 hours prior to separation and that it is possible to increase twice as well as decrease twice the sample amount usually used without any change in retention ratios. The implementation of flow-rate gradients for elution of the starch granules did not improve the resolution of flow-rate gradients for evolution of the starch granules did not improve the resolution of the peaks A and B compared with generally used constant flow-rate.
Permanent Link: http://hdl.handle.net/11104/0059575