0162255 - UIACH-O 20010009 RIV NL eng J - Journal Article
Horká, Marie - Willimann, T. - Blum, M. - Nording, P. - Friedl, Z. - Šlais, Karel
Capillary isoelectric focusing with UV-induced fluorescence detection.
Journal of Chromatography A. Roč. 916, 1-2 (2001), s. 65-71. ISSN 0021-9673. E-ISSN 1873-3778
R&D Projects: GA AV ČR IAA4031901
Institutional research plan: CEZ:AV0Z4031919
Keywords : isoelectric focusing * detection * electrophoresis
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 2.793, year: 2001

Low-molecular-mass fluorescent compounds excitable in the near UV region with suitable acidobasic and electrophoretic properties are suggested as isoelectric point (pI) markers for isoelectric focusing (IEF) with UV excited fluorometric detection and properties of new UV-induced fluorescent pI markers are given. The PI values of 18 new pI markers determined independently of IEF methods range from 2.1 to 10.3 The examples of separation of new pI markers together with derivatized proteins by capillary IEF with photometric or fluorometric detection are presented.
Permanent Link: http://hdl.handle.net/11104/0059574