0162248 - UIACH-O 200075 RIV CZ eng J - Journal Article
Radko, S. P. - Šťastná, Miroslava - Buzas, S. - Kingsley, D. - Chrambach, A.
Charge heterogeneity of commercial, red-shifted recombinant green fluorescent protein, revealed by capillary zone electrophoresis under nondenaturing conditions.
Analytical Biochemistry. Roč. 274, - (2000), s. 146-148. ISSN 0003-2697. E-ISSN 1096-0309
Institutional research plan: CEZ:AV0Z4031919
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 1.976, year: 2000

The demonstration in electrophoresis of charge isomerism of rEGFP nonderivatized with SDS presents an illustration for the fact that apparent homogeneity of a protein in SDS-PAGE only refers to the molecular weight of the gene product and is blind to posttranslational modifications or other sources of isomerism. In this respect, CZE combined with the Ferguson plot analysis of recombinant proteins, compared to conventional SDS-PAGE.
Permanent Link: http://hdl.handle.net/11104/0059567