0157661 - PAU-O 20033048 RIV GB eng J - Journal Article
Shionoya, M. - Matsubayashi, H. - Asahina, Masako - Kuniyoshi, H. - Nagata, S. - Riddiford, L. M. - Kataoka, H.
Molecular cloning of the prothoracicotropic hormone from the tobacco hornworm, Manduca sexta.
Insect Biochemistry and Molecular Biology. Roč. 33, č. 8 (2003), s. 795-801. ISSN 0965-1748. E-ISSN 1879-0240
Grant - others:National Science Foundation(US) IBN9005202; National Science Foundation(US) IBN9514187; JP(CZ) Ministry of Education; GA-(CZ) Science; Sports and Culture of Japan(CZ) Grant-in-Aid for Scientific Research on Priority Areas08276101
Institutional research plan: CEZ:AV0Z6022909
Keywords : prothoracicotropic hormone * PTTH * Manduca sexta
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.358, year: 2003

A cDNA encoding a putative precursor of prothoracicotropic hormone (PTTH) from the tobacco hornworm, Manduca sexta, was isolated and sequenced. This clone contains an open reading frame encoding a 226-amino acid prepropeptide hormone. The deduced amino acid sequence is composed of a signal sequence, a precursor domain and a mature hormone and shows similarities to the other PTTHs that have been cloned from closely related lepidopteran species, Bombyx mori, Samia cynthia ricini, Antheraea peryni, and Hyalophora cecropia. The recombinant putative Manduca PTTH produced in E. coli was biologically active, both causing a larval molt in neck-ligated Manduca 4th instar larvae (ED50=50 pM) and the adult molt of diapausing Manduca pupae (ED50=79 pM), but was unable to stimulate molting of debrained Bombyx pupae.
Permanent Link: http://hdl.handle.net/11104/0055099