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Coprodiagnosis of Hammondia heydorni in dogs by PCR based on ITS 1 rRNA: differentiation from morphologically indistinguishable oocysts of Neospora caninum

    1.
    SYSNO ASEP0157604
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JOstatní články
    NázevCoprodiagnosis of Hammondia heydorni in dogs by PCR based on ITS 1 rRNA: differentiation from morphologically indistinguishable oocysts of Neospora caninum
    Tvůrce(i) Šlapeta, Jan Roger (PAU-O)
    Koudela, Břetislav (PAU-O)
    Votýpka, Jan (CZ)
    Modrý, David (PAU-O)
    Hořejš, R. (CZ)
    Lukeš, Julius (PAU-O)
    Zdroj.dok.Veterinary Journal. - : Elsevier - ISSN 1090-0233
    Roč. 163, č. 2 (2002), s. 147-154
    Poč.str.8 s.
    Jazyk dok.eng - angličtina
    Země vyd.GB - Velká Británie
    Klíč. slovaHammondia heydorni ; Neospora caninum ; coprodiagnosis
    Vědní obor RIVEG - Zoologie
    CEPIAA6022903 GA AV ČR - Akademie věd
    CEZAV0Z6022909 - PAU-O, BC-A
    AnotaceHammondia heydorni is thought to be a non-pathogenic coccidian parasite of dogs that is closely related to Neospora caninum, an important parasite of cattle and dogs. Oocysts of these two species are morphologically indistinguishable from each other. A population of 2240 dogs in the Czech Republic was screened for the presence of H. heydorni/N. caninum oocysts and five (0.22), represented by five of 3135 faecal samples (0.16), were positive. The internal transcribed spacer 1 region of the rRNA gene (ITS1) from two isolates were cloned and the DNA sequences were identical with those of the ITS1 of H. heydorni. Based on the rRNA sequences available for H. heydorni and related coccidia, the primer pair JS4-JS5 was designed to amplify the 3' end of the small subunit (SSU) rRNA gene and ITS1 of H. heydorni. When tested on DNA extracted from a variety of parasites, the primers amplified a specific 267 bp fragment in our isolates only. The presence of DNA equivalent to 10 oocysts was sufficient for the amplification of the ITS1. We present a PCR-based diagnostic method as the only fast and reliable method for the diagnosis of H. heydorni in dogs.
    PracovištěParazitologický ústav (do r. 2005)
    KontaktKateřina Homrová,katerina.homrova@paru.cas.cz , Tel.: 387 775 406
    Rok sběru2003

Number of the records: 1  

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