Marec, František (ENTU-I) Sahara, K. (JP) Traut, W. (DE)
Source Title
Annales de Genetique
- ISSN 0003-3995
Roč. 44, č. 1 (2001), s. 131
Number of pages
1 s.
Action
European Cytogenetics Conference /3./
Event date
07.07.2001-10.07.2001
VEvent location
Paris
Country
FR - France
Event type
EUR
Language
eng - English
Country
FR - France
Keywords
Telomere ; sex chromosomes ; chromosome fragments
Subject RIV
EB - Genetics ; Molecular Biology
CEZ
AV0Z5007907 - ENTU-I, BC-A
Annotation
Broken ends in three sex-chromosome mutant lines of the flour moth, Ephestia kuehniella , were examined by fluorescence in situ hybridization (FISH) with the insect telomere probe (TTAGG)n on spread preparations of pachytene oocytes. No hybridization signals were found at cut ends of Z-chromosome fragments and at one end of the deficient W chromosome. This implies that the broken ends had not been restored by a synthesis of telomeric DNA de novo or by another mechanism. In spite of the telomere loss, the broken ends of sex chromosomes evidently did not cause chromosome instability, cell cycle arrest or apoptosis. In contrast, they persisted in ASF lines for more than 50 generations (more than 11 years).
Workplace
Institute of Entomology (until 2005)
Contact
Helena Musilová, musilova@entu.cas.cz, Tel.: 387 775 216
Year of Publishing
2002
Number of the records: 1
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