Isolation of chromosomes from Picea abies L. and their analysis by flow cytometry

Number of the records: 1

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SYSNO ASEP	0107473
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Isolation of chromosomes from Picea abies L. and their analysis by flow cytometry
Title	Izolace chromozómů smrku a jejich třídění pomocí průtokové cytometrie
Author(s)	Überall, I. (CZ) Vrána, Jan (UEB-Q)_{RID, ORCID} Bartoš, Jan (UEB-Q)_{RID, ORCID} Šmerda, J. (CZ) Doležel, Jaroslav (UEB-Q)_{RID, ORCID} Havel, L. (CZ)
Source Title	Biologia Plantarum. - : Ústav experimentální botaniky AV ČR, v. v. i. - ISSN 0006-3134 Roč. 48, č. 2 (2004), s. 199-203
Number of pages	5 s.
Language	eng - English
Country	CZ - Czech Republic
Keywords	Vicia faba ; composite map
Subject RIV	ED - Physiology
R&D Projects	GA522/03/0354 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z5038910 - UEB-Q
Annotation	A high-yield method for preparation of suspensionsof intact Norway spruce [Picea abies (L.) Karst] chromosomes was developed for the first time. To accumulate meristem root tip cells at metaphase, actively growing roots were subjected to subsequent treatments with 0.625 mM hydroxyurea for 18 h and after 8 h recovery in distilled water with 0.05
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2005

Number of the records: 1