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Magnetic hydrophilic methacrylate-based polymer microspheres designed for polymerase chain reactions applications
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SYSNO ASEP 0105806 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Magnetic hydrophilic methacrylate-based polymer microspheres designed for polymerase chain reactions applications Title Magnetické hydrofilní polymerní mikročástice na bázi methakrylátu pro použití v PCR Author(s) Španová, A. (CZ)
Horák, Daniel (UMCH-V) RID, ORCID
Soudková, E. (CZ)
Rittich, B. (CZ)Source Title Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. - : Elsevier - ISSN 1570-0232
Roč. 800, 1-2 (2004), s. 27-32Number of pages 6 s. Action International Symposium on Separations in the Bioscience /3./ Event date 13.05.2003-18.05.2003 VEvent location Moscow Country RU - Russian Federation Event type WRD Language eng - English Country NL - Netherlands Keywords polymerase chain reaction inhibition ; magnetic microspheres ; methacrylates Subject RIV BM - Solid Matter Physics ; Magnetism R&D Projects GA202/02/1361 GA ČR - Czech Science Foundation (CSF) GA525/02/0287 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z4050913 - UMCH-V Annotation Magnetic hydrophilic non-porous P(HEMA-co-EDMA), P(HEMA-co-GMA) and PGMA microspheres were prepared by dispersion (co)polymerization of 2-hydroxyethyl methacrylate (HEMA) and ethylene dimethacrylate (EDMA) or glycidyl methacrylate (GMA) in the presence of several kinds of magnetite. It was found that some components used in the preparation of magnetic carriers interfered with polymerase chain reaction (PCR). Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2005
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