Number of the records: 1
Grb2 and the non-T cell activation linker NTAL constitute a Ca(2+)-regulating signal circuit in B lymphocytes
- 1.
SYSNO ASEP 0105347 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Grb2 and the non-T cell activation linker NTAL constitute a Ca(2+)-regulating signal circuit in B lymphocytes Title Grb2 a NTAL (non-T cell activation linker) tvoří signální okruh regulující Ca(2+) v B lymfocytech Author(s) Stork, B. (DE)
Engelke, M. (DE)
Frey, J. (DE)
Hořejší, Václav (UMG-J) RID
Hamm-Baarke, A. (DE)
Schraven, B. (DE)
Kurosaki, T. (JP)
Wienands, J. (DE)Source Title Immunity. - : Elsevier - ISSN 1074-7613
Roč. 21, č. 5 (2004), s. 681-691Number of pages 10 s. Language eng - English Country US - United States Keywords NTAL ; Grb2 ; lymphocyte Subject RIV EC - Immunology R&D Projects LN00A026 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z5052915 - UMG-J Annotation Activation of the B cell antigen receptor triggers phosphorylation of cytoplasmic and transmembrane adaptor proteins such as SLP-65 and NTAL, respectively. Specific phosphoacceptor sites in SLP-65 serve as docking sites for Ca(2+)-mobilizing enzymes Btk and PLC-gamma2. Phosphorylated NTAL recruits the Grb2 linker, but downstream signaling cascades are unclear. We now show that receptor-induced tyrosine phosphorylation of NTAL and concomitant Grb2 complex formation critically modulate the Ca(2+) response without affecting SLP-65 and PLC-gamma2 phosphorylation. Grb2 turned out to play a negative regulatory role, which appears to be eliminated upon binding to NTAL. This allows for a sustained release of intracellular Ca(2+) and is mandatory for subsequent entry of Ca(2+) from extracellular sources. Thus, elevation of Ca(2+) is regulated by at least two signaling modules, the B cell-specific Ca(2+) initiation complex comprising SLP-65, Btk, and PLC-gamma2 and the more ubiquitously expressed NTAL/Grb2 complex, which acts as an amplifier by switching off inhibitory elements Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2010
Number of the records: 1