| SYSNO ASEP | 0105273
|
|---|
| Druh ASEP | J - Článek v odborném periodiku
|
|---|
| Zařazení RIV | J - Článek v odborném periodiku
|
|---|
| Poddruh J | Ostatní články
|
|---|
| Název | Reverse effect of indomethacin on the immunosuppressive activity of boar seminal immunosuppressive fraction
|
|---|
| Překlad názvu | Opačný efekt indomethacinu na imunosupresivní aktivitu kančí semenné imunosupresivní frakce
|
|---|
| Tvůrce(i) | Veselský, Leopold (UMG-J)
Dostál, Jaromír (UZFG-Y)
Kraus, Marek (UMG-J)
Pěknicová, Jana (UMG-J)
Holáň, Vladimír (UMG-J)
Zajícová, Alena (UMG-J)
Jonáková, Věra (UMG-J)
Železná, Blanka (UMG-J)
|
|---|
| Zdroj.dok. | Animal Reproduction Science. - : Elsevier
- ISSN 0378-4320
č. 71 (2002), s. 111-123
|
|---|
| Poč.str. | 13 s.
|
|---|
| Jazyk dok. | eng - angličtina
|
|---|
| Země vyd. | US - Spojené státy americké
|
|---|
| Klíč. slova | Indomethacin ; Antibody response ; Seminal immunosuppressive fraction
|
|---|
| Vědní obor RIV | CE - Biochemie
|
|---|
| CEP | KSK5011112 GA AV ČR - Akademie věd
|
|---|
| GV524/96/K162 GA ČR - Grantová agentura ČR
|
|---|
| GA303/01/0615 GA ČR - Grantová agentura ČR
|
|---|
| NI6883 GA MZd - Ministerstvo zdravotnictví
|
|---|
| Anotace | By measuring bromodeoxyuridine incorporation into replicated DNA, we found that immunosuppressive fraction of boar seminal plasma (ISF) inhibited mitogen-stimulated lymphocyte proliferation; pretreatment with monoclonal antibody to ISF or indomethacin reversed the ISF effect. Besides, we followed the ability of indomethacin or monoclonal antibody to ISF to reverse ISF-induced suppression of antibody production by measuring antibody titers in the blood sera of mice immunized with keyhole limpet hemocyanin using ELISA. Recently we have identified ISF as a complex of the major seminal glycoproteins PSP I and PSP II. PSP II is the part that is responsible for immunosuppressive properties of the complex. To learn whether the ISF immunosuppressive effect is associated with its protein or saccharide part, we examined the deglycosylated PSP II for its antiproliferative effect on mitogen-stimulated mouse lymphocytes. The results suggest that deglycosylation of PSP II did not affect its antiproliferative activity. This suggest that PSP II immunosuppressive properties are associated with the protein and not the saccharide part of the molecule
|
|---|
| Pracoviště | Ústav molekulární genetiky
|
|---|
| Kontakt | Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
|
|---|
| Rok sběru | 2005
|
|---|
