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High-affinity binding of tumor supressor protein p53 and HMGB1 protein to hemicatenated DNA loops
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SYSNO ASEP 0104679 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title High-affinity binding of tumor supressor protein p53 and HMGB1 protein to hemicatenated DNA loops Title Vysoce afinitní vazba nádorově supresorového proteinu p53 a HMGB1 s hemikatenátovými smyčkami DNA Author(s) Štros, Michal (BFU-R) RID, ORCID
Muselíková Polanská, Eva (BFU-R)
Pospíšilová, Š. (CZ)
Strauss, F. (FR)Source Title Biochemistry. - : American Chemical Society - ISSN 0006-2960
Roč. 43, č. 22 (2004), s. 7215-7225Number of pages 11 s. Language eng - English Country US - United States Keywords p53 ; HMGB1 ; DNA hemicatenane Subject RIV BO - Biophysics R&D Projects IAA5004105 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) GA301/02/0952 GA ČR - Czech Science Foundation (CSF) KSK4055109 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z5004920 - BFU-R Annotation We have observed that chromatin architectural protein HMGB1 and tumor suppressor protein p53 could bind with high affinity to a novel DNA structure that forms a DNA loop maintained at its base by a hemicatenane (termed hcDNA). The affinity of p53 for hcDNA is 40-fold higher (Kd ~ 0.5 nM) than for its natural specific binding sites within its target genes (Mdm2 promoter). Binding of p53 to hcDNA remains detectable in the presence of up to ~4 orders of magnitude of mass excess of competitor linear DNA, suggesting a high specificity of the interaction. p53 displays a higher affinity for hcDNA than for DNA minicircles (lacking functional p53-specific binding sequence) with a size similar to that of the loop within the hcDNA, indicating that the extreme affinity of p53 for hcDNA is likely due to binding of the protein to the hemicatenane. Only the full-length p53 can form stable ternary complexes with hcDNA and HMGB1. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2005
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