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Investigations of the supercoil-selective DNA binding of wild type p53 suggest a novel mechanism for controlling p53 function
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SYSNO ASEP 0104632 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Investigations of the supercoil-selective DNA binding of wild type p53 suggest a novel mechanism for controlling p53 function Title Výsledky studií selektivní vazby proteinu p53 standardního typu k nadšroubovicové DNA naznačují nový mechanismus regulace funkce p53 Author(s) Fojta, Miroslav (BFU-R) RID, ORCID
Pivoňková, Hana (BFU-R) ORCID, RID
Brázdová, Marie (BFU-R) RID, ORCID
Němcová, Kateřina (BFU-R)
Paleček, Jan (BFU-R)
Vojtěšek, B. (CZ)Source Title European Journal of Biochemistry - ISSN 0014-2956
Roč. 271, č. 9 (2004), s. 3865-3876Number of pages 12 s. Language eng - English Country GB - United Kingdom Keywords monoclonal antibodies ; p53 latency ; redox state Subject RIV BO - Biophysics R&D Projects GA204/02/0734 GA ČR - Czech Science Foundation (CSF) GA301/02/0831 GA ČR - Czech Science Foundation (CSF) NC7574 GA MZd - Ministry of Health (MZ) IBS5004009 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) Annotation We studied supercoil-selective binding of bacterially expressed p53 using modulation of activity of the p53 DNA-binding domains by oxidation of cysteine residues and/or by antibodies mapping to epitopes at the protein C-terminus. Blocking the CTDBS with antibody caused reduced p53 to bind equally to sc and linear or relaxed circular DNA lacking p53CON, while oxidized p53 in the absence of antibody restored selective scDNA binding. Our results support a hypothesis that alterations to DNA topology may be a component of the complex cellular regulatory mechanisms that control the switch between latent and active p53 following cellular stress. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2005
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