Number of the records: 1
Microanalysis of DNA by stripping transfer voltammetry
- 1.
SYSNO ASEP 0104622 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Microanalysis of DNA by stripping transfer voltammetry Title Mikroanalýza DNA pomocí rozpouštěcí přenosové voltametrie Author(s) Jelen, František (BFU-R) RID
Kouřilová, Alena (BFU-R)
Pečinka, Petr (BFU-R) RID
Paleček, Emil (BFU-R) RID, ORCIDSource Title Bioelectrochemistry. - : Elsevier - ISSN 1567-5394
Roč. 63, 1-2 (2004), s. 249-252Number of pages 4 s. Language eng - English Country CH - Switzerland Keywords DNA determination ; determination of purine bases ; linear sweep voltammetry Subject RIV BO - Biophysics R&D Projects GA203/02/0422 GA ČR - Czech Science Foundation (CSF) IAA1163201 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) IBS5004107 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z5004920 - BFU-R Annotation A cathodic stripping transfer voltammetric procedure for trace determination of DNA and its components is described. The method is based on the DNA acid hydrolysis with subsequent electrochemical determination of released purine bases. In the first step, DNA is hydrolyzed for 30 min in 0.5 M perchloric acid at 75 degreesC. The electrochemical step involves generation of Cu(I)-purine base complex on a mercury electrode surface, transfer of electrode with accumulated complex into supporting electrolyte where voltammetric measurement is performed. Analysis is carried out in 14-mul drop volume (two-electrode connection) or in 30-mul drop (three-electrode connection) on a platinum plate, which is used as a counter electrode. Blank electrolyte contains 0.05 M borate buffer, pH 9.2 with 6.3 muM Cu(II). We could observe voltammetric signal at hydrolyzed nucleosides, nucleotides, ODN, and DNA containing purine bases. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2005
Number of the records: 1