0104484 - PAU-O 20043062 RIV US eng J - Journal Article
Xiao, L. - Ryan, U. M. - Graczyk, T. K. - Limor, J. - Li, L. - Kombert, M. - Junge, R. - Sulaiman, I. M. - Zhou, L. - Arrowood, M. J. - Koudela, Břetislav - Modrý, David - Lal, A. A.
Genetic diversity of Cryptosporidium spp. in captive reptiles.
[Genetická diversita Cryptosporidium spp. u chovaných plazů.]
Applied and Environmental Microbiology. Roč. 70, č. 2 (2004), s. 891-899. ISSN 0099-2240. E-ISSN 1098-5336
R&D Projects: GA ČR GA524/00/P015
Institutional research plan: CEZ:AV0Z6022909
Keywords : Cryptosporidium * reptiles * genetic diversity
Subject RIV: EG - Zoology
Impact factor: 3.810, year: 2004

The genetic diversity of Cryptosporidium in reptiles was analyzed by PCR-restriction fragment length polymorphism and sequence analysis of the small subunit rRNA gene. A total of 123 samples were analyzed, of which 48 snake samples, 24 lizard samples, and 3 tortoise samples were positive for Cryptosporidium. Nine different types of Cryptosporidium were found, including Cryptosporidium serpentis, Cryptosporidium desert monitor genotype, Cryptosporidium muris, Cryptosporidium parvum bovine and mouse genotypes, one C. serpentis-like parasite in a lizard, two new Cryptosporidium spp. in snakes, and one new Cryptosporidium sp. in tortoises. C. serpentis and the desert monitor genotype were the most common parasites and were found in both snakes and lizards, whereas the C. muris and C. parvum parasites detected were probably the result of ingestion of infected rodents

Genetická diversita zástupců r. Cryptosporidium u plazů byla analyzována prostřednictvím PCR-restriction fragment length polymorphismu a sekvenační analýzou ssu rRNA genu. 123 vzorků bylo vyšetřeno, z toho 48 z hadů, 24 z ještěrů, 3 ze želv bylo pozitivní na Cryptosporidium. Bylo nalezeno 9 odlišných typů Cryptosporidium, včetně Cryptosporidium serpentis, Cryptosporidium-desert monitor genotype, Cryptosporidium muris, Cryptosporidium parvum bovine a mouse genotypes, jeden C. serpentis-like izolát u ještěra, dva nové Cryptosporidium spp. u hadů a jeden nový Cryptosporidium sp. u želv. C. serpentis a desert monitor genotype byly nejčastější parazité, nalézaní jak u hadů tak u ještěrů. C. muris a C. parvum byly pravděpodobně jen pasážovány z potravy
Permanent Link: http://hdl.handle.net/11104/0011750