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The application of image cytometry to viability assessment in dual fluorescence-stained fish spermatozoa

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    SYSNO ASEP0102927
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JOstatní články
    TitleThe application of image cytometry to viability assessment in dual fluorescence-stained fish spermatozoa
    TitlePoužití obrazové cytometrie k posouzení životnosti duálně fluorescenčně barvených spermií ryb
    Author(s) Flajšhans, Martin (UZFG-Y)
    Cosson, J. (FR)
    Rodina, Marek (UZFG-Y)
    Linhart, Otomar (UZFG-Y)
    Source TitleCell Biology International. - : Elsevier - ISSN 1065-6995
    Roč. 28, č. 12 (2004), s. 955-959
    Number of pages5 s.
    Languageeng - English
    CountryUS - United States
    Keywordsimage cytometry ; dual fluorescent ; staining
    Subject RIVED - Physiology
    R&D ProjectsME 638 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    GA524/03/0178 GA ČR - Czech Science Foundation (CSF)
    KSK6005114 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
    CEZAV0Z5045916 - UZFG-Y
    AnnotationThe viability of spermatozoa has been assessed using SYBR 14 staining for DNA of living cells and propidium iodide staining for DNA of degenerate cells. This dual staining was performed on four fish species (Siberian sturgeon, Acipenser baerii; common carp, Cyprinus carpio, tench, Tinca tinca and wels, Silurus glanis) and the proportions of live and dead spermatozoa were assessed by epifluorescence microscopy and image cytometry.
    WorkplaceInstitute of Animal Physiology and Genetics
    ContactJana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
    Year of Publishing2005
Number of the records: 1  

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