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Confocal stereology and image analysis: methods for estimating geometrical characteristics of cells and tissues from three-dimensional confocal images
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SYSNO ASEP 0102529 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Confocal stereology and image analysis: methods for estimating geometrical characteristics of cells and tissues from three-dimensional confocal images Title Stereologie a analýza obrazu v konfokální mikroskopii: metody pro odhad geometrických charakteristik buněk a tkání s využitím trojrozměrných konfokálních obrazů Author(s) Kubínová, Lucie (FGU-C) RID, ORCID
Janáček, Jiří (FGU-C) RID, ORCID
Karen, Petr (FGU-C)
Radochová, Barbora (FGU-C) RID, ORCID
Difato, Francesco (FGU-C)
Krekule, Ivan (FGU-C)Source Title Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408
Roč. 53, Suppl.1 (2004), s. S47-S55Number of pages 9 s. Language eng - English Country CZ - Czech Republic Keywords confocal microscopy ; image analysis ; stereology Subject RIV EA - Cell Biology R&D Projects GA304/01/0257 GA ČR - Czech Science Foundation (CSF) GA310/02/1470 GA ČR - Czech Science Foundation (CSF) KJB6011309 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) KJB5039302 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z5011922 - FGU-C Annotation A short review of confocal stereology and three-dimensional image analysis is presented, pointing out the achievements accomplished in this field by the Department of Biomathematics (Institute of Physiology, Prague). One of the methods of confocal stereology, the fakir method for surface area estimation, developed by this laboratory, is described. Methods for automatic measurement of geometrical characteristics of microscopical structures, based on 3-D image processing or surface triangulation, are discussed and compared with interactive stereological methods. Three-dimensional reconstruction programs and software implementation of stereological and digital methods as well as their practical applications are presented Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2005
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