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A novel staining method for quantification and 3D visualisation of capillaries and muscle fibres
- 1.0102528 - FGU-C 20040051 RIV IT eng J - Journal Article
Čebašek, V. - Kubínová, Lucie - Ribarič, S. - Eržen, I.
A novel staining method for quantification and 3D visualisation of capillaries and muscle fibres.
[Nová metoda barvení pro kvantifikaci a trojrozměrnou vizualizaci kapilár a svalových vláken.]
European Journal of Histochemistry. Roč. 48, č. 2 (2004), s. 151-157. ISSN 1121-760X. E-ISSN 2038-8306
Grant - others:SI - CZ(CZ) KONTAKT 001/2001
Institutional research plan: CEZ:AV0Z5011922
Keywords : capillaries * confocal microscopy * immunohistochemistry
Subject RIV: EA - Cell Biology
Impact factor: 0.845, year: 2004
The aim of this study was to introduce a combined fluorescent staining that clearly demonstrates capillaries and distinguishes them from the basal lamina of muscle fibres in skeletal muscle tissue. The triple staining with CD31, Griffonia (Bandeira) simplicifolia lectin (GSL I) and laminin efficiently distinguishes vascular endothelium from the basal lamina of skeletal muscle fibres in physiological and pathological conditions. The presented triple staining method has several advantages, which facilitate quantitative analysis of the capillary network, and its relation to individual muscle fibres
Zavedli jsme kombinované fluorescenční barvení, které jasně vizualizuje kapiláry a odlišuje je od bazální laminy svalových vláken v kosterním svalu. Tato metoda barvení usnadňuje kvantitativní analýzu kapilární sítě ve vztahu k jednotlivým svalovým vláknům
Permanent Link: http://hdl.handle.net/11104/0009876
Number of the records: 1