Characterization of spirochetal isolates from arthropods collected in South Moravia, Czech Republic, using fatty acid methyl esters analysis

Čechová, L.; Durnová, E.; Šikutová, Silvie; Halouzka, Jiří; Němec, M.

doi:https://dx.doi.org/10.1016/j.jchromb.2004.05.014

Number of the records: 1

Characterization of spirochetal isolates from arthropods collected in South Moravia, Czech Republic, using fatty acid methyl esters analysis

1.

SYSNO ASEP	0101771
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Characterization of spirochetal isolates from arthropods collected in South Moravia, Czech Republic, using fatty acid methyl esters analysis
Title	Charakterizace spirochét izolovaných z členovců na jižní Moravě, Česká republika, analýzou methylesterů mastných kyselin
Author(s)	Čechová, L. (CZ) Durnová, E. (CZ) Šikutová, Silvie (UBO-W)_{RID, SAI, ORCID} Halouzka, Jiří (UBO-W)_SAI Němec, M. (CZ)
Source Title	Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. - : Elsevier - ISSN 1570-0232 Roč. 808, č. 2 (2004), s. 249-254
Number of pages	6 s.
Language	eng - English
Country	NL - Netherlands
Keywords	spirochetes ; arthropods ; fatty acid methyl esters
Subject RIV	EE - Microbiology, Virology
R&D Projects	GA206/03/0726 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z6093917 - UBO-W
DOI	https://doi.org/10.1016/j.jchromb.2004.05.014
Annotation	Aim of this study was to evaluate cellular fatty acid analysis for characterization of spirochetes. Strains were isolated from arthropods collected in South Moravia, Czech Republic. Fatty acid methyl esters (FAME) profile was determined for five Borrelia burgdorferi sensu lato (s.l.) strains isolated from Ixodes ricinus ticks, one "Spironema culicis" strain recovered from mosquito Culex pipiens and seven spirochetal strains (not identified yet) isolated from mosquitoes and blackflies. Analysis was performed using a gas chromatography column in conjunction with Microbial Identification System Sherlock (MIDI Inc., Newark, DE, USA). Results obtained on the basis of cluster analysis of FAME profiles showed, that the B. burgdorferi sensu lato isolates could be well separated from other spirochetal isolates. We recommended method used in this study as a useful tool for preliminary identification of spirochetes isolated from ticks and dipterans.
Workplace	Institute of Vertebrate Biology
Contact	Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524
Year of Publishing	2005

Number of the records: 1