0100808 - UOCHB-X 20043034 RIV GB eng J - Journal Article
Birkuš, Gabriel - Rejman, Dominik - Otmar, Miroslav - Votruba, Ivan - Rosenberg, Ivan - Holý, Antonín
The substrate activity of (S)-9-[3-hydroxy-(2-phosphonomethoxy)propyl]adenine diphosphate toward DNA polymerases alpha, delta and epsilon.
[Substrátová aktivita difosfátu (S)-9-[3-hydroxy-(2-phosphonomethoxy)propyl]adeninu k DNA-polymerasám alfa, delta a epsílon.]
Antiviral Chemistry and Chemotherapy. Roč. 15, - (2004), s. 23-33. ISSN 0956-3202
R&D Projects: GA ČR GA203/01/1166; GA AV ČR IBS4055109
Institutional research plan: CEZ:AV0Z4055905
Keywords : HPMPA * cellular DNA polymerases * HPMPC
Subject RIV: CC - Organic Chemistry

In this study the substrate potency of (S)-9-[(3-hydroxy-2-phosphonomethoxy)-propyl]adenine diphosphate (HPMPApp) toward DNA polymerases was examined. It was found that efficiency of HPMPApp incorporation decreased in the order pol epsilon > pol delta and was from 5.4 to 23 fold lower than that of dATP. These observations might contribute to cellular toxicity of HPMPA and explain its antiviral activity against polyomavirus

Byla studována účinnost difosfátu (S)-9-[3-hydroxy-(2-phosphonomethoxy)propyl]adeninu (HPMPApp) jako substrátu DNA-polymeras. Míra inkorporace HPMPApp byla vyšší pro pol epsílon než pro pol delta ale 5,4 až 23 krát nižší než inkorporace dATP, což je nejspíše příčinou celulární toxicity a virostatické aktivity vůči polyomavirům
Permanent Link: http://hdl.handle.net/11104/0008294