Number of the records: 1  

Optimalization of artificial propagation in European catfish, Silurus glanis L

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    SYSNO ASEP0100606
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JOstatní články
    TitleOptimalization of artificial propagation in European catfish, Silurus glanis L
    TitleZlepšení technologie úmělého výtěru sumce velkého, Silurus glanis L
    Author(s) Linhart, Otomar (UZFG-Y)
    Gela, David (UZFG-Y)
    Rodina, Marek (UZFG-Y)
    Kocour, Martin (UZFG-Y)
    Source TitleAquaculture. - : Elsevier - ISSN 0044-8486
    Roč. 235, - (2004), s. 619-632
    Number of pages14 s.
    Languageeng - English
    CountryUS - United States
    Keywordseggs ; sprematozoa ; aquaculture
    Subject RIVED - Physiology
    R&D ProjectsOK 187 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    GA524/03/0178 GA ČR - Czech Science Foundation (CSF)
    CEZAV0Z5045916 - UZFG-Y
    AnnotationThe present work optimizes procedures for artificial insemination of European catfish, with goals to increase collection of sperm and hatching rate. We address technical aspects of immobilizing solution, activation solution, process of insemination, activation of gametes and elimination of eggs stickness with a practical solution for artificial propagation in hatcheries. Spermaiation was stimulated and could be sustained over a period of 1 month after weekly carp pituitary (CP) injection of 5 mg kg –1 b.w.Males produced significantly the largest quantity of sperm, 0,12-0,13. 109 spermatozoa kg –1 b.w., after third and fourth injections. The best IS for sperm was determined as solution containing 17 mM NaCl, 5 mM Tris-HCl, pH 7. The ratio of 1:2 rather than ratio 1:1 in the control batch (volume of activation solution in the control batchmillimetres/grams of eggs) increased the hatchability up to 68%. The highest success of 82-88% from four individual females were found for sperm number of 800-80.000 spermatozoa egg-1.
    WorkplaceInstitute of Animal Physiology and Genetics
    ContactJana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
    Year of Publishing2005
Number of the records: 1  

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