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Classical and molecular approaches as a powerful tool for the characterization of rumen polycentric fungi
- 1.0100601 - UZFG-Y 20043020 RIV CZ eng J - Journal Article
Fliegerová, Kateřina - Hodrová, Blanka - Voigt, K.
Classical and molecular approaches as a powerful tool for the characterization of rumen polycentric fungi.
[Klasický a molekularní přístup k charakterizaci bachorových polycentrických hub.]
Folia Microbiologica. Roč. 49, č. 2 (2004), s. 157-164. ISSN 0015-5632. E-ISSN 1874-9356
R&D Projects: GA ČR GA523/96/0103; GA AV ČR KSK5020115
Institutional research plan: CEZ:AV0Z5045916
Keywords : rumen polycentric fungi
Subject RIV: EE - Microbiology, Virology
Impact factor: 1.034, year: 2004
Ribosomal ITS1 and ITS2 fragments from 8 isolates of polycentric rumen anaerobic fungi were PCR-amplified and sequenced; the sequences obtained were aligned with published data and phylogenetic analyses were performed.
Fragmenty ribosomalni DNA ITS1 a ITS2 z osmi izolatu anaerobnich bachorovych polycentrickych hub byly amplifikovany, sekvenovany a fylogeneticky analyzovany. Vysledky teto analyzy podporovane morfologickym pozorovanim potvrdily, ze sekvencni analyzu ITS fragmentu lze vyuzit k zretelnemu rozdeleni dvou rodu polycentrickych hub Orpinomyces a Anaeromyces a ze tyto anaerobni chytridiomycety tvori monofyletickou skupinu separovanou od aerobnich chytridiomycet.
Permanent Link: http://hdl.handle.net/11104/0008099
Number of the records: 1