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Molecular cloning and expression of the pyranose 2-oxidase cDNA from Trametes ochracea MB49 in Escherichia coli

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    0100422 - MBU-M 20040111 RIV DE eng J - Journal Article
    Večerek, B. - Marešová, Helena - Kočanová, Marcela - Kyslík, Pavel
    Molecular cloning and expression of the pyranose 2-oxidase cDNA from Trametes ochracea MB49 in Escherichia coli.
    [Molekulární klonování a exprese pyranózy 2-oxidázy cDNA z Trametes ochracea MB49 v Escherichia coli.]
    Applied Microbiology and Biotechnology. Roč. 64, č. 4 (2004), s. 525-530. ISSN 0175-7598. E-ISSN 1432-0614
    Institutional research plan: CEZ:AV0Z5020903
    Keywords : p2o * lb * m9-based mineral
    Subject RIV: EE - Microbiology, Virology
    Impact factor: 2.358, year: 2004

    cDNA of a structural gene encoding pyranose 2-oxidase (P2O) from Trametes ochracea strain MB49 was cloned into E. coli strain BL21(DE3) on a multicopy plasmid under the control of trc promoter.

    cDNA strukturálního genu kódujícího pyranosa 2-oxidasu z Trametes ochracea MB49 byla naklonována do E. coli BL21(DE3) na multikopiový plasmid za trc promotor.
    Permanent Link: http://hdl.handle.net/11104/0007924

     
     
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