The relationship between the modular structure of eukaryotic-like serine/threonine protein kinase StkP of Streptococcus pneumoniae and its function

0100398 - MBU-M 20049087 RIV ES eng C - Conference Paper (international conference)
Pallová, Petra - Přenosilová, Lenka - Nováková, Linda - Branny, Pavel
The relationship between the modular structure of eukaryotic-like serine/threonine protein kinase StkP of Streptococcus pneumoniae and its function.
[Vztah mezi modulární strukturou serin/threonin proteinkinasy eukaryontního typu StkP z Streptococcus pneumoniae a její funkcí.]
Euresco Conference Bacterial Neural Networks /2004./. San Feliu de Quixols, 2004, s. 156.
[Bacterial Neural Networks, Euro Conference on Inter- and Intracellular Signalling, and Global Regulation in Bacteria /2004./. San Feliu de Guixols (ES), 08.05.2004-14.05.2004]
Institutional research plan: CEZ:AV0Z5020903
Keywords : stkp * c-terminal
Subject RIV: EE - Microbiology, Virology

In order to determine a cellular localization of StkP we prepared its epitope-labelled form expressed in S. pneumoniae. The full-length form of StkP was detected with monoclonal antibody in the membrane fraction. In vitro autophosphorylation assays confirmed this finding and phosphorylated product of corresponding mass was detected in the membranes. These results clearly showed that StkP is a membrane-associated protein.

Byla určena buněčná lokalizace proteinkinasy StkP pomocí integrace do genomu S. pneumoniae jejího genu označeného epitopem. Nativní forma StkP byla detekována monoklonální protilátkou v membránové frakci. Tento výsledek byl ověřen in vitro autofosforylací a fosforylovaný produkt odpovídající velikosti byl detekován v membránách.
Permanent Link: http://hdl.handle.net/11104/0007900