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Factors influencing DNA expansion in the course of polymerase chain reaction
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SYSNO ASEP 0080841 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Factors influencing DNA expansion in the course of polymerase chain reaction Title Faktory ovlivňující expanzi DNA během polymerázové řetězové reakce Author(s) Vondrušková, Jitka (BFU-R)
Pařízková, Naďa (BFU-R)
Kypr, Jaroslav (BFU-R) RIDSource Title Nucleosides Nucleotides & Nucleic Acids. - : Taylor & Francis - ISSN 1525-7770
Roč. 26, č. 1 (2007), s. 65-82Number of pages 18 s. Language eng - English Country US - United States Keywords DNA expansion ; PCR ; microsatellites Subject RIV BO - Biophysics R&D Projects NM7634 GA MZd - Ministry of Health (MZ) CEZ AV0Z50040702 - BFU-R (2007-2013) Annotation We performed more than 3,500 PCRs under various conditions with more than 400 DNA fragments of 4-150 nucleotides in length. We found out that repetitiveness of the primary structure was mostly found to be necessary but not sufficient for the expansion. (A+T)-rich fragments expand better than (G+C)-rich ones and pyrimidine-rich fragments expand better than purine-rich fragments. Terminal nucleotides and the fragment length also are important for the expansion. examples are presented when relatively small alterations of the DNA primary structure caused a dramatic change in the expansion. The present work has implications for pathological expansions of microsatellites in the human genome as well as regarding the genome evolution in general. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2007
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