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Volume visualization of large biological tissue specimens
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SYSNO ASEP 0080620 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Volume visualization of large biological tissue specimens Title Objemová vizualizace velkých biologických tkáňových vzorků Author(s) Čapek, Martin (FGU-C) RID, ORCID
Kubínová, Lucie (FGU-C) RID, ORCID
Janáček, Jiří (FGU-C) RID, ORCID
Hána, K. (CZ)
Smrčka, P. (CZ)Source Title Analysis of Biomedical Signals and Images. - Brno : VUTIUM Press, 2006 / Jan J. ; Kozumplík J. ; Provazník I. - ISBN 80-214-3152-0 Pages s. 230-232 Number of pages 3 s. Action BIOSIGNAL 2006 /18./ Event date 28.06.2006-30.06.2006 VEvent location Brno Country CZ - Czech Republic Event type EUR Language eng - English Country CZ - Czech Republic Keywords confocal microscopy ; volume reconstruction Subject RIV JC - Computer Hardware ; Software R&D Projects LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) IAA100110502 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) IAA500200510 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) GA304/05/0153 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50110509 - FGU-C (2005-2011) Annotation We apply volume reconstruction for visualization of a biological specimen greater than the field of view of a confocal laser scanning microscope. Prior to the volume reconstruction, large specimens are cut into thin physical slices. The first step of volume reconstruction is acquisition of digital volume images (spatial tiles which overlap) from all studied physical slices. The second step is horizontal merging of overlapping spatial tiles of the same physical slice using a registration algorithm based on a mutual information and translation. The third reconstruction step is vertical merging of digital volumes of successive physical slices using an elastic registration algorithm based on B-splines. The resulting large digital volumes are visualized by a VolumePro hardware board that provides volume rendering in real-time. In this paper we show a reconstruction of a chick embryonic kidney. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2007
Number of the records: 1