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Approaches to Design Whole Cell Biosensor for PCB Analysis Based on Optical Detection
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SYSNO ASEP 0054882 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Approaches to Design Whole Cell Biosensor for PCB Analysis Based on Optical Detection Title Celobuněčný biosensor pro optickou detekci PCB Author(s) Gavlasová, Pavla (UCHP-M)
Kuncová, Gabriela (UCHP-M) RID, SAI
Macková, M. (CZ)Source Title Scientific Programme.. - Gliwice : -, 2006 Pages s. 27-28 Number of pages 2 s. Action Trends in Ecotoxicology. Emerging Pollutants: Endocrine Disrupting Substances, Pharmaceutical and Personal Care Products Event date 03.04.2006-04.04.2006 VEvent location Gliwice Country PL - Poland Event type EUR Language eng - English Country PL - Poland Keywords pcbs ; biosensor ; cells Subject RIV CE - Biochemistry R&D Projects GA104/05/2637 GA ČR - Czech Science Foundation (CSF) GA203/06/0583 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z40720504 - UCHP-M (2005-2011) Annotation This study is carried out to investigate simple and cheaper method for detection of polychlorinated biphenyls in environmental samples. Our approach is based on optical detection of yellow intermediates (?_max = 398 nm) produced by silica entrapped cells of /Pseudomonas species /2 (P2).In our experiments the cells of P2 strain were immobilized into the sol-gel in the absence or in the presence of biphenyl (for improve of reusability of immobilized cells). Biosensor was exposed to a commercial PCBs mixture Delor103 (D103).The results show that sol-gel entrapped cells are able to produce yellow intermediates (?_max = 398 nm) in presence of D103 in the range of 1-20 mg.L-1. The selectivity of the method for some selected PCB congeners was also tested. It was determined for two PCB congeners (2,4,4´-trichlorobiphenyl and 2,4´,5-trichlorobiphenyl). Workplace Institute of Chemical Process Fundamentals Contact Eva Jirsová, jirsova@icpf.cas.cz, Tel.: 220 390 227 Year of Publishing 2007
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