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Methods for compensation of the light attenuation with depth of images captured by a confocal microscope
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SYSNO ASEP 0049753 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Methods for compensation of the light attenuation with depth of images captured by a confocal microscope Title Metody kompenzace útlumu jasu s hloubkou obrazů získaných konfokálním mikroskopem Author(s) Čapek, Martin (FGU-C) RID, ORCID
Janáček, Jiří (FGU-C) RID, ORCID
Kubínová, Lucie (FGU-C) RID, ORCIDSource Title Microscopy Research and Technique - ISSN 1059-910X
Roč. 69, č. 8 (2006), s. 624-635Number of pages 12 s. Language eng - English Country US - United States Keywords image enhancement ; confocal microscopy ; histogram warping Subject RIV JC - Computer Hardware ; Software R&D Projects LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) IAA100110502 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) IAA600110507 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) IAA500200510 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) KJB6011309 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) GA304/05/0153 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50110509 - FGU-C (2005-2011) Annotation A confocal laser scanning microscope (CLSM) enables us to capture images from a biological specimen in different depths and obtain a series of precisely registered fluorescent images. But images captured from deep layers of the specimen may be darker than images from the topmost layers due to light loss distortions. This effect causes difficulties in subsequent analysis of biological objects. We propose a solution using two approaches: either an online method working already during image acquisition, or an offline method assisting as a post-processing step. In the online method the gain value of a photomultiplier tube of a CLSM is controlled according to the difference of mean image intensities between the reference and currently acquired image. The offline method consists of two stages. In the first stage a standard histogram improving brightness and contrast is created from all images in the series. In the second stage image histograms are warped according to this standard histogram Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2007
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