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A new, sensitive method for enzyme kinetic studies of scarce glucosides
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SYSNO ASEP 0042442 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title A new, sensitive method for enzyme kinetic studies of scarce glucosides Title Nová citlivá metoda pro studium enzymové kinetiky vzácných glukozidů Author(s) Mazura, Pavel (BFU-R)
Fohlerová, Radka (BFU-R)
Brzobohatý, Břetislav (BFU-R) RID, ORCID
Kiran, N.S. (IN)
Janda, L. (CZ)Source Title Journal of Biochemical and Biophysical Methods - ISSN 0165-022X
Roč. 68, č. 1 (2006), s. 55-63Number of pages 9 s. Language eng - English Country NL - Netherlands Keywords Amplex Ultra Red reagent ; beta-glucosidase ; zeatin-O-glukozid Subject RIV BO - Biophysics R&D Projects GA203/02/0865 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50040507 - BFU-R (2005-2011) Annotation The maize beta-glucosidase Zm-p60.1 is important for the regulation of plant development through its role in the targeted release of cytokinins from cytokinin-O-glucosides, their inactive storage forms. Enzyme kinetics studies using these scarce substrates close to physiological concentrations are difficult. We developed a glucose assay using a system comprising three enzymes - beta-glucosidase, glucose oxidase and horseradish peroxidase, with Amplex Ultra Red reagent. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2007
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