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Detection of volatile compounds emitted by Pseudomonas aeruginosa using selected ion flow tube mass spectrometry
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SYSNO ASEP 0030950 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Detection of volatile compounds emitted by Pseudomonas aeruginosa using selected ion flow tube mass spectrometry Title Detekce těkavých látek emitovaných bakteriemi Pseudomonas aeruginosa pomocí hmotnostní spektrometrie v proudové trubici s vybranými ionty Author(s) Carroll, W. (GB)
Lenney, W. (GB)
Wang, T. (GB)
Španěl, Patrik (UFCH-W) RID, ORCID
Alcock, A. (GB)
Smith, D. (GB)Source Title Pediatric Pulmonology - ISSN 8755-6863
Roč. 39, č. 5 (2005), s. 452-456Number of pages 5 s. Language eng - English Country US - United States Keywords pseudomonas aeruginosa ; hydrogen cyanide ; biomarkers ; SIFT-MS Subject RIV CF - Physical ; Theoretical Chemistry CEZ AV0Z40400503 - UFCH-W (2005-2011) Annotation Pseudomonas aeruginosa (PA) is associated with a distinctive smell produced by a combination of volatile compounds (VCs). Selected ion flow tube mass spectrometry (SIFT-MS) provides a novel and rapid methodology for rapid, accurate detection of trace quantities (parts per billion; ppb) of VCs in air. We studied the VCs produced by different isolates of PA cultures in vitro from patients with cystic fibrosis. Twenty-one patients with cystic fibrosis provided sputum and cough swab samples for culture. These were used to inoculate blood agar (BA) and Pseudomonas-selective media (PSM). These plates were incubated for 48 hr at 37 degrees C inside sealed plastic bags. The air surrounding the samples after 48 hr (headspace) was analyzed using SIFT-MS. PA growth was commonly associated with the production of significant quantities of VCs, notably hydrogen cyanide gas (HCN). This was detectable in the headspace of 15/22 of PA-positive samples. Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2006
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