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Cytotoxic effect of soil cyanobacterial extracts to mammal cell lines YAC-1 and WEHI
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SYSNO ASEP 0029096 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Cytotoxic effect of soil cyanobacterial extracts to mammal cell lines YAC-1 and WEHI Title Cytotoxický efekt extraktů půdních sinic k liniím savčích buněk YAC-1 A WEHI Author(s) Hrouzek, Pavel (MBU-M) ORCID
Kopecký, Jiří (MBU-M) ORCID
Salát, Jiří (PAU-O)
Maršálek, Blahoslav (BU-J) ORCID, SAI
Lukešová, Alena (UPB-H)Source Title Czech Phycology - ISSN 1213-3434
Roč. 5, - (2005), s. 79-90Number of pages 12 s. Language eng - English Country CZ - Czech Republic Keywords cytotoxic effect ; soil cyanobacterial extracts ; mammal cell lines YAC-1 and WEHI Subject RIV EH - Ecology, Behaviour CEZ AV0Z60050516 - BU-J (2005-2011) AV0Z60660521 - UPB-H, BC-A (2005-2011) Annotation The cytotoxic effects of ten methanol extracts obtained from soil filamentous cyanobacteria (Anabaena, Calothrix, Nodularia, Cylindrospermum, Tolypothrix, Trichormus) were studied. Two different mammal cell lines (YAC-1, WEHI) were selected for cytotoxicity testing using the MTT test and flow-cytometry (FC). For comparison, the brine shrimp assay (using Artemia as a testing organism) was performed. The composition of extracts was studied using HPLC-MS. Both MTT and FC found the cytotoxic effect in 6 of 10 tested cyanobacterial extracts, but these results did not correlate with those obtained by brine shrimp assay. In Anabaena torulosa,Cylindrospermum sp. the most severe damage was recorded by both methods. In cells treated by the extract of A. torulosa, 100% inhibition was found already after 60-minute exposure. Induction of necrosis was revealed by FC, since all tested cells were marked by propidium iodide indicating disruption of the cellular membrane. Workplace Institute of Soil Biology (until 2005) Contact Ivana Chytková, chytkova@upb.cas.cz, Tel.: 387 775 753 Year of Publishing 2006
Number of the records: 1