Fuková, Iva (ENTU-I) Nguyen, Petr (ENTU-I) Marec, František (ENTU-I)
Source Title
Genome. - : Canadian Science Publishing
- ISSN 0831-2796
Roč. 48, - (2005), s. 1083-1092
Number of pages
10 s.
Language
eng - English
Country
CA - Canada
Keywords
CGH ; codling moth ; FISH
Subject RIV
EB - Genetics ; Molecular Biology
R&D Projects
IAA6007307 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ
AV0Z50070508 - ENTU-I, BC-A (2005-2011)
Annotation
We performed a detailed karyotype analysis in the codling moth, Cydia pomonella, the key pest of pome fruit in the temperate regions of the world. The karyotype consisted of 2n = 56 chromosomes. In pachytene nuclei, a curious NOR (nucleolar organizer region) bivalent was observed. It carried 2 nucleoli, each associated with one end of the bivalent as confirmed by FISH with an 18S ribosomal DNA, which detected 2 clusters of rRNA genes. Pachytene oocytes showed a sex chromosome bivalent (WZ). Molecular differentiation of the sex chromosomes was studied using genomic in situ hybridization (GISH) and comparative genomic hybridization (CGH). GISH detected the W chromosome by strong binding of the female-derived DNA probe. With CGH, both the female-derived probe and male-derived probe evenly bound to the W chromosome. This suggested that the W chromosome is predominantly composed of repetitive DNA sequences occurring scattered in other chromosomes but accumulated in the W chromosome.
Workplace
Institute of Entomology (until 2005)
Contact
Helena Musilová, musilova@entu.cas.cz, Tel.: 387 775 216
Year of Publishing
2006
Number of the records: 1
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