0028463 - ÚMG 2006 RIV US eng J - Journal Article
Backman, M. - Machoň, Ondřej - Mygland, L. - van den Bout, C. J. - Zhong, W.M. - Taketo, M.M. - Krauss, S.
Effects of canonical Wnt signaling on dorso-ventral specification of the mouse telencephalon.
[Efekt dráhy Wnt na dorso-ventrální specifikaci telencephalonu.]
Developmental Biology. Roč. 279, č. 1 (2005), s. 155-168. ISSN 0012-1606. E-ISSN 1095-564X
R&D Projects: GA ČR(CZ) GA204/04/1358
Institutional research plan: CEZ:AV0Z50520514
Keywords : beta-catenin * telencephalon * progenitor cell identity
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 5.234, year: 2005

Wnt signaling is involved in numerous processes during vertebrate CNS development. In this study, we used conditional Cre/loxP system in mouse to ablate or activate beta-catenin in the telencephalon in two time windows: before and after the onset of neurogenesis. We show that beta-catenin mediated Wnt signals are required to maintain the molecular identity of the pallium. Inactivation of beta-catenin in the telencephalon before neurogenesis results in downregulated expression of dorsal markers Emx1, Emx2 and Ngn2, and in ectopic up-regulation of ventral markers Gsh2, Mash1 and Dlx2 in the pallium. Thus, our data suggest that canonical Wnt signals are involved in maintaining the identity of the pallium by controlling expression of dorsal markers and by suppressing ventral programs from being activated in pallial progenitor cells.

Dráha Wnt se účastní řady procesů během vývoje centrální nervové soustavy (CNS). Použili jsme kondicionální systém CRE/loxP abychom inaktivovali gen pro beta-katenin před a po začátku neurogeneze. Ukázalo se, že dráha Wnt udržuje identitu buněk v CNS kontrolou exprese dorsálních markerů a supresí ventrálních markerů.
Permanent Link: http://hdl.handle.net/11104/0118401