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Changes in key enzymes of viral-RNA biosynthesis in chloroplasts from PVY and TMV infected tobacco plants
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SYSNO ASEP 0028411 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Changes in key enzymes of viral-RNA biosynthesis in chloroplasts from PVY and TMV infected tobacco plants Title Změny regulačních enzymů vedoucích k biosyntéze virové RNA v chloroplastech z PVY a TMV infikovaných rostlin tabáku Author(s) Šindelářová, Milada (UEB-Q)
Šindelář, Luděk (UEB-Q)
Wilhelmová, Naděžda (UEB-Q) RID, ORCID
Procházková, Dagmar (UEB-Q) RIDSource Title Biologia Plantarum. - : Ústav experimentální botaniky AV ČR, v. v. i. - ISSN 0006-3134
Roč. 49, - (2005), s. 471-474Number of pages 4 s. Language eng - English Country NL - Netherlands Keywords tobacco ; virus ; chloroplast Subject RIV CE - Biochemistry R&D Projects GA522/02/0708 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50380511 - UEB-Q (2005-2011) Annotation Effects of the infection with tobacco mosaic virus (TMV) and potato virus Y (PVY) on chloroplasts from susceptible tobacco plants were determined. Changes in ribonucleases (RNases), phosphomonoesterase (PME), phosphodiesterase (PDE), glucose-6-phosphate dehydrogenase (G6P DH), 6-phosphogluconate dehydrogenase (6PG DH), glucokinase (GK), and fructokinase (FK) activities in thylakoid/envelope and stroma fractions were studied. Slight increase in the activities of PME, PDE, G6P DH and 6PG DH of thylakoid/envelope fraction as well as of RNases, PME, PDE, G6P DH, 6PG DH, GK and FK of stroma fraction was found in chloroplasts isolated from leaf tissues infected with PVY. Infection with TMV produced higher increase in enzymes activities in chloroplasts; especially, PME, G6P DH and 6PG DH in fraction of thylakoid/envelope, and RNases, PME, PDE, G6P DH, 6PG DH, and GK in stroma fraction. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2006
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