Cell death induced by taxanes in breast cancer cells: cytochrome C is released in resistant but not in sensitive cells
1.
SYSNO ASEP
0027651
Document Type
J - Journal Article
R&D Document Type
Journal Article
Subsidiary J
Ostatní články
Title
Cell death induced by taxanes in breast cancer cells: cytochrome C is released in resistant but not in sensitive cells
Title
Buněčná smrt u buněk nádorů prsu indukovaná taxany: cytochrom C se vylévá u odolných, ale ne vucitlivých buněk
Author(s)
Ehrlichová, Marie (UMG-J) Koc, Michal (UMG-J) Truksa, Jaroslav (UMG-J) Naďová, Zuzana (UMG-J) Václavíková, R. (CZ) Kovář, Jan (UMG-J)
Source Title
Anticancer Research. - : International Institute of Anticancer Research
- ISSN 0250-7005
Roč. 25, 6B (2005), s. 4215-4224
Number of pages
10 s.
Language
eng - English
Country
GR - Greece
Keywords
paclitaxel ; cell death ; breast cancer cells
Subject RIV
EB - Genetics ; Molecular Biology
R&D Projects
NL7567 GA MZd - Ministry of Health (MZ)
CEZ
AV0Z50520514 - UMG-J (2005-2011)
Annotation
The aim of the study was to contribute to our understanding of the mechanisms responsible for the resistance of breast cancer cells to taxanes. Approximately 300-fold higher concentrations of the taxanes were required to induce death in resistant NCI-ADR-RES cells than in sensitive MDA-MB-435 cells. Cell death induced by the taxanes in both cells was preceded by the accumulation of cells in the G2/M-phase. Neither cell type produced any DNA fragmentation (DNA ladder) typical of regular apoptosis. The death induced by the taxanes in the studied breast cancer cells can be characterized as an apoptosis-like death, including caspase-3 and caspase-9 activation but not oligonucleosomal DNA fragmentation. However, the mechanisms of death induction by the taxanes in sensitive MDA-MB-435 cells and resistant NCI-ADR-RES cells differ. Cytochrome c is released from the mitochondria in resistant but not in sensitive cells.
