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The HLDA8 blind panel: findings and conclusions
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SYSNO ASEP 0027620 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title The HLDA8 blind panel: findings and conclusions Title Slepý panel HLDA8: poznatky a závěry Author(s) Swart, B. (AU)
Salganik, M.P. (US)
Wand, M.P. (AU)
Tinckam, K. (US)
Milford, E.L. (US)
Drbal, Karel (UMG-J)
Angelisová, Pavla (UMG-J) RID
Hořejší, Václav (UMG-J) RID
Macardle, P. (AU)
Bailey, S. (AU)
Hollemweguer, E. (US)
Hodge, G. (AU)
Nairn, J. (AU)
Millard, D. (AU)
Dagdeviren, A. (TR)
Dandie, G.W. (AU)
Zola, H. (AU)Source Title Journal of Immunological Methods. - : Elsevier - ISSN 0022-1759
Roč. 305, č. 1 (2005), s. 75-83Number of pages 9 s. Language eng - English Country NL - Netherlands Keywords CD molecules ; leukocyte antigen Subject RIV EC - Immunology CEZ AV0Z5052915 - UMG-J Annotation There were over 600 antibodies submitted to HLDA8, with many of unknown specificity. Of these, 101 antibodies were selected for a blind panel study that also included 5 negative controls and 27 positive controls of known CD specificity making a total of 133 antibodies in the final panel. Of the 101 unknowns, 31 antibodies were identified during the course of this blind panel study as being specific for known molecules and included some specific for MHC class II antigens, CD45 isoforms and the Dombrock antigen. Several antibody pairs among those in the blind panel were found to have very similar staining patterns and were therefore compared by immunohistochemical and/or Western blot analyses for identity. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2006
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