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Chromosome sorting in tetraploid wheat and its potential for genome analysis
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SYSNO ASEP 0026518 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Chromosome sorting in tetraploid wheat and its potential for genome analysis Title Třídění chromozómů u tetraploidní pšenice a možnosti pro analýzu jejího genomu Author(s) Kubaláková, Marie (UEB-Q) RID
Kovářová, Pavlína (UEB-Q)
Suchánková, Pavla (UEB-Q) RID
Čihalíková, Jarmila (UEB-Q) RID
Bartoš, Jan (UEB-Q) RID, ORCID
Lucretti, S. (IT)
Watanabe, N. (JP)
Kianian, S. F. (US)
Doležel, Jaroslav (UEB-Q) RID, ORCIDSource Title Genetics. - : Genetics Society of America - ISSN 0016-6731
Roč. 170, - (2005), s. 823-829Number of pages 7 s. Language eng - English Country US - United States Keywords Flow cytometry ; chromosome sorting ; wheat Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA522/03/0354 GA ČR - Czech Science Foundation (CSF) GA521/04/0607 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50380511 - UEB-Q (2005-2011) Annotation This study evaluates the potential of flow cytometry for chromosome sorting in durum wheat (Triticum turgidum Desf. var. durum,). Histograms of fluorescence intensity obtained after the analysis of DAPI-stained chromosomes consisted of three peaks. Of these, one represented chromosome 3B, a small peak corresponded to chromosomes 1A and 6A, and a large peak represented the remaining eleven chromosomes. Flow karyotyping in double ditelosomic lines of durum wheat revealed that the lines facilitated sorting of any arm of the wheat A- and B-genome chromosomes. We have demonstrated that large insert libraries can be created from DNA purified using flow cytometry. This study considerably expands the potential of flow cytogenetics for use in wheat genomics and opens the possibility of sequencing the genome of this important crop one chromosome arm at a time. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2006
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