Unique mitochondrial genome structure in diplonemids, the sister group of kinetoplastids
1.
SYSNO ASEP
0025875
Document Type
J - Journal Article
R&D Document Type
Journal Article
Subsidiary J
Ostatní články
Title
Unique mitochondrial genome structure in diplonemids, the sister group of kinetoplastids
Title
Jedinečná struktura mitochondriálního genomu u diplonemid, sesterské skupiny kinetoplastid
Author(s)
Marande, W. (CA) Lukeš, Julius (PAU-O) Burger, G. (CA)
Source Title
Eukaryotic Cell
- ISSN 1535-9778
Roč. 4, č. 6 (2005), s. 1137-1146
Number of pages
10 s.
Language
eng - English
Country
US - United States
Keywords
Diplonema ; kinetoplast ; trans-splicing
Subject RIV
EB - Genetics ; Molecular Biology
R&D Projects
IAA5022302 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ
AV0Z60220518 - PAU-O, BC-A (2005-2011)
Annotation
We describe the mitochondrial genome and the mitochondrial ultrastructure of Diplonema papillatum - a member of diplonemid flagellates, which are the sister group of kinetoplastids. DNA is evenly distributed throughout the mitochondrion rather than compacted into a kinetoplast body. Molecular studies distinguish numerous small mitochondrial chromosomes that fall into two size classes, 6 and 7 kbp. In vivo, these molecules are of monomeric circular topology and relaxed conformation. Remarkably, the cox1 gene (and probably other mitochondrial genes) is fragmented, with separate gene pieces encoded on different chromosomes. Generation of the contiguous cox1 mRNA requires trans-splicing, the precise mechanism of which remains to be determined. Taken together, the mitochondrial gene/genome structure of Diplonema is not only different from that of kinetoplastids but unique among eukaryotes as a whole.
