Number of the records: 1
Modulation of murine cellular immune response and cytokine production by salivary gland lysate of three sand fly species
- 1.
SYSNO ASEP 0025688 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Modulation of murine cellular immune response and cytokine production by salivary gland lysate of three sand fly species Title Modulace myší buněčné imunitní odpovědi a produkce cytokinů působením lyzátu slinných žláz tří druhů flebotomů Author(s) Rohoušová, Iva (UMG-J)
Volf, P. (CZ)
Lipoldová, Marie (UMG-J) RIDSource Title Parasite immunology. - : Wiley - ISSN 0141-9838
Roč. 12, č. 27 (2005), s. 469-473Number of pages 5 s. Language eng - English Country GB - United Kingdom Keywords cytokine production ; Lutzomyia ; Phlebotomus Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA310/03/1381 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z5052915 - UMG-J Annotation Saliva of sand flies (Diptera: Phlebotominae) plays an important role in transmission of Leishmania parasites by modulating host immune response. However, because of the different protein compositions of saliva, the immunomodulatory effects may vary among sand fly species. We have therefore analysed the immunomodulation effects of salivary gland lysate (SGL) of three different sand flies. Spleen cells from BALB/c mice were incubated with SGL of Phlebotomus papatasi, P. sergenti or Lutzomyia longipalpis. Concanavalin A-stimulated lymphocyte proliferation was suppressed with SGLs of all three sand fly species and all SGL doses tested. SGL also markedly inhibited IFN-gamma production in all intervals tested (reduced up to 31%) and to a lesser degree impaired production of the IL-2 and IL-4. Despite some species-specific differences in the intensity of immunomodulatory effects, saliva of all sand fly species modulated cell proliferation as well as cytokine production in a similar way. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2006
Number of the records: 1