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Apoptosis Related Human Wharton's Jelly-Derived Stem Cells Differentiation into Osteoblasts, Chondrocytes, Adipocytes and Neural-like Cells-Complete Transcriptomic Assays
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SYSNO ASEP 0573910 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Apoptosis Related Human Wharton's Jelly-Derived Stem Cells Differentiation into Osteoblasts, Chondrocytes, Adipocytes and Neural-like Cells-Complete Transcriptomic Assays Author(s) Stefanska, K. (PL)
Němcová, Lucie (UZFG-Y) RID
Blatkiewitz, M. (PL)
Pienkowski, W. (PL)
Rucinski, M. (PL)
Zabel, M. (PL)
Mozdziak, P. (US)
Podhorska-Okolow, M. (PL)
Dziegiel, P. (PL)
Kempisty, B. (PL)Article number 10023 Source Title International Journal of Molecular Sciences. - : MDPI
Roč. 24, č. 12 (2023)Number of pages 27 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords Wharton´s jelly ; mesenchymal stem cells ; RNAseq Subject RIV EB - Genetics ; Molecular Biology OECD category Developmental biology R&D Projects EF15_003/0000460 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UZFG-Y - RVO:67985904 UT WOS 001017505600001 EID SCOPUS 85163946958 DOI 10.3390/ijms241210023 Annotation Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) exhibit multilineage differentiation potential, adhere to plastic, and express a specific set of surface markers-CD105, CD73, CD90. Although there are relatively well-established differentiation protocols for WJ-MSCs, the exact molecular mechanisms involved in their in vitro long-term culture and differentiation remain to be elucidated. In this study, the cells were isolated from Wharton's jelly of umbilical cords obtained from healthy full-term deliveries, cultivated in vitro, and differentiated towards osteogenic, chondrogenic, adipogenic and neurogenic lineages. RNA samples were isolated after the differentiation regimen and analyzed using an RNA sequencing (RNAseq) assay, which led to the identification of differentially expressed genes belonging to apoptosis-related ontological groups. ZBTB16 and FOXO1 were upregulated in all differentiated groups as compared to controls, while TGFA was downregulated in all groups. In addition, several possible novel marker genes associated with the differentiation of WJ-MSCs were identified (e.g., SEPTIN4, ITPR1, CNR1, BEX2, CD14, EDNRB). The results of this study provide an insight into the molecular mechanisms involved in the long-term culture in vitro and four-lineage differentiation of WJ-MSCs, which is crucial to utilize WJ-MSCs in regenerative medicine. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2024 Electronic address https://www.mdpi.com/1422-0067/24/12/10023
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