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Investigation of Protein Corona Formed around Biologically Produced Gold Nanoparticles
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SYSNO ASEP 0562630 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Investigation of Protein Corona Formed around Biologically Produced Gold Nanoparticles Author(s) Pourali, Parastoo (MBU-M)
Neuhoferová, Eva (MBU-M) ORCID
Dzmitruk, Volha (BTO-N)
Benson, Veronika (MBU-M) RID, ORCIDArticle number 4615 Source Title Materials. - : MDPI
Roč. 15, č. 13 (2022)Number of pages 22 s. Language eng - English Country CH - Switzerland Keywords biologically produced gold nanoparticles ; hard protein corona ; capping agent ; Fusarium oxysporum Subject RIV EE - Microbiology, Virology OECD category Microbiology Subject RIV - cooperation Institute of Biotechnology R&D Projects LM2018127 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF18_046/0015974 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Research Infrastructure Czech-BioImaging II - 90129 - Ústav molekulární genetiky AV ČR, v. v. i. Method of publishing Open access Institutional support MBU-M - RVO:61388971 ; BTO-N - RVO:86652036 UT WOS 000822164200001 EID SCOPUS 85133535239 DOI 10.3390/ma15134615 Annotation Although there are several research articles on the detection and characterization of protein corona on the surface of various nanoparticles, there are no detailed studies on the formation, detection, and characterization of protein corona on the surface of biologically produced gold nanoparticles (AuNPs). AuNPs were prepared from Fusarium oxysporum at two different temperatures and characterized by spectrophotometry, Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and energy-dispersive X-ray spectroscopy (EDS). The zeta potential of AuNPs was determined using a Zetasizer. AuNPs were incubated with 3 different concentrations of mouse plasma, and the hard protein corona was detected first by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then by electrospray liquid chromatography-mass spectrometry (LC-MS). The profiles were compared to AuNPs alone that served as control. The results showed that round and oval AuNPs with sizes below 50 nm were produced at both temperatures. The AuNPs were stable after the formation of the protein corona and had sizes larger than 86 nm, and their zeta potential remained negative. We found that capping agents in the control samples contained small peptides/amino acids but almost no protein(s). After hard protein corona formation, we identified plasma proteins present on the surface of AuNPs. The identified plasma proteins may contribute to the AuNPs being shielded from phagocytizing immune cells, which makes the AuNPs a promising candidate for in vivo drug delivery. The protein corona on the surface of biologically produced AuNPs differed depending on the capping agents of the individual AuNP samples and the plasma concentration. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2023 Electronic address https://www.mdpi.com/1996-1944/15/13/4615
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