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Monophyly of diverse Bigyromonadea and their impact on phylogenomic relationships within stramenopiles
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SYSNO ASEP 0557960 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Monophyly of diverse Bigyromonadea and their impact on phylogenomic relationships within stramenopiles Author(s) Cho, A. (CA)
Tikhonenkov, D. V. (RU)
Hehenberger, Elizabeth (BC-A) ORCID, RID
Karnkowska, A. (PL)
Mylnikova, A. (RU)
Keeling, P.J. (CA)Number of authors 6 Article number 107468 Source Title Molecular Phylogenetics and Evolution. - : Elsevier - ISSN 1055-7903
Roč. 171, JUN (2022)Number of pages 14 s. Publication form Online - E Language eng - English Country US - United States Keywords rna gene sequence ; aggregative multicellularity ; heterotrophic flagellate ; developayella-elegans ; spec-nov ; phytophthora ; tree ; evolution ; ultrastructure ; classification ; Bigyromonadea ; Developea ; Pirsoniales ; Oomycetes ; Phagoheterotrophs ; Stramenopile ; Phylogenomics ; Aggregation ; Zoospores ; Single-cell transcriptome Subject RIV CE - Biochemistry OECD category Biochemistry and molecular biology Method of publishing Open access Institutional support BC-A - RVO:60077344 UT WOS 000796815500003 EID SCOPUS 85127804129 DOI 10.1016/j.ympev.2022.107468 Annotation Stramenopiles are a diverse but relatively well-studied eukaryotic supergroup with considerable genomic information available (Sibbald and Archibald, 2017). Nevertheless, the relationships between major stramenopile subgroups remain unresolved, in part due to a lack of data from small nanoflagellates that make up a lot of the genetic diversity of the group. This is most obvious in Bigyromonadea, which is one of four major stramenopile subgroups but represented by a single transcriptome. To examine the diversity of Bigyromonadea and how the lack of data affects the tree, we generated transcriptomes from seven novel bigyromonada species described in this study: Develocauda condao n. gen. n. sp., Develocanicus komovi n. gen. n. sp., Develocanicus vyazemskyi n. sp., Cubaremonas variflagellatum n. gen. n. sp., Pirsonia chemainus nom. prov., Feodosia pseudopoda nom. prov., and Koktebelia satura nom. prov. Both maximum likelihood and Bayesian phylogenomic trees based on a 247 genematrix recovered a monophyletic Bigyromonadea that includes two diverse subgroups, Developea and Pirsoniales, that were not previously related based on single gene trees. Maximum likelihood analyses show Bigyromonadea related to oomycetes, whereas Bayesian analyses and topology testing were inconclusive. We observed similarities between the novel bigyromonad species and motile zoospores of oomycetes in morphology and the ability to self-aggregate. Rare formation of pseudopods and fused cells were also observed, traits that are also found in members of labyrinthulomycetes, another osmotrophic stramenopiles. Furthermore, we report the first case of eukaryovory in the flagellated stages of Pirsoniales. These analyses reveal new diversity of Bigyromonadea, and altogether suggest their monophyly with oomycetes, collectively known as Pseudofungi, is the most likely topology of the stramenopile tree. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2023 Electronic address https://www.sciencedirect.com/science/article/pii/S1055790322000811?via%3Dihub
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