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Resistant Genes and Multidrug-Resistant Bacteria in Wastewater: A Study of Their Transfer to the Water Reservoir in the Czech Republic
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SYSNO ASEP 0555953 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Resistant Genes and Multidrug-Resistant Bacteria in Wastewater: A Study of Their Transfer to the Water Reservoir in the Czech Republic Author(s) Stachurová, T. (CZ)
Sýkorová, N. (CZ)
Semerád, Jaroslav (MBU-M) ORCID, RID
Malachová, K. (CZ)Article number 147 Source Title Life. - : MDPI
Roč. 12, č. 2 (2022)Number of pages 16 s. Language eng - English Country CH - Switzerland Keywords antibiotic-resistance ; treatment plants ; pseudomonas-aeruginosa ; diversity ; association ; prevalence ; abundance ; traits ; sewage ; pcr ; antibiotic resistance gene ; beta-lactam resistance ; tetracycline resistance ; multidrug resistance ; wastewater ; wastewater treatment plant ; water environment Subject RIV EH - Ecology, Behaviour OECD category Environmental sciences (social aspects to be 5.7) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000767201600001 EID SCOPUS 85124036083 DOI 10.3390/life12020147 Annotation Wastewater is considered the most serious source of the spread of antibiotic resistance in the environment. This work, therefore, focuses on the fate and spread of antibiotic resistance genes (ARGs) in wastewater and the monitoring of multidrug-resistant strains. ARGs were monitored in the nitrification and sedimentation tanks of the wastewater treatment plant (WWTP) and in the dam into which this WWTP flows, at various times. The highest relative abundance was found for the blaTEM > tetW > blaNDM-1 > vanA resistance genes, respectively. An increased concentration of tetracycline (up to 96.00 ng/L) and ampicillin (up to 19.00 ng/L) was found in water samples compared to other antibiotics detected. The increased incidence of seven ARGs and four antibiotics was observed in the November and December sampling times. Isolated ampicillin-resistant strains showed a high degree of resistance to ampicillin (61.2% of the total isolates had a minimum inhibitory concentration (MIC) >= 20 mg/mL). In 87.8% of isolates, out of the total number, the occurrence of two or more ARGs was confirmed. These multidrug-resistant strains were most often identified as Aeromonas sp. This strain could represent a significant role in the spread of multidrug resistance through wastewater in the environment. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2023 Electronic address https://www.mdpi.com/2075-1729/12/2/147
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