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The role of G-Quadruplex DNA in Paraspeckle formation in cancer
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SYSNO ASEP 0555745 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The role of G-Quadruplex DNA in Paraspeckle formation in cancer Author(s) Bhatt, U. (AU)
Kretzmann, A. (AU)
Guédin, A. (FR)
Ou, A. (AU)
Kobelke, S. (AU)
Bond, C. (AU)
Evans, C. (AU)
Hurley, L. (US)
Mergny, Jean-Louis (BFU-R) ORCID, RID
Iyer, K. (AU)
Fox, A.H. (AU)
Smith, N. (AU)Number of authors 12 Source Title Biochimie. - : Elsevier - ISSN 0300-9084
Roč. 190, NOV 2021 (2021), s. 124-131Number of pages 8 s. Publication form Print - P Language eng - English Country FR - France Keywords long noncoding rna ; gene-expression ; stress-response ; down-regulation ; breast-cancer ; lncrna neat1 ; progression OECD category Biochemistry and molecular biology R&D Projects EF15_003/0000477 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support BFU-R - RVO:68081707 UT WOS 000701671300011 EID SCOPUS 85111735735 DOI 10.1016/j.biochi.2021.07.008 Annotation Paraspeckles are RNA-protein structures within the nucleus of mammalian cells, capable of orchestrating various biochemical processes. An overexpression of the architectural component of paraspeckles, a long non-coding RNA called NEAT1 (Nuclear Enriched Abundant Transcript 1), has been linked to a variety of cancers and is often associated with poor patient prognosis. Thus, there is an accumulating interest in the role of paraspeckles in carcinogenesis, however there is a limited understanding of how NEAT1 expression is regulated. Here, we demonstrate that both nuclear G-quadruplex (G4) and paraspeckle formation are significantly increased in a human breast cancer cell line compared to nontumorigenic breast cells. Moreover, we identified and characterized G4-forming sequences within the NEAT1 promoter and demonstrate stabilization of G4 DNA with a G4-stabilizing small molecule results in a significant alteration in both paraspeckle formation and NEAT1 expression. This G4-mediated alteration of NEAT1 at both the transcriptional and post-transcriptional levels was evident in U2OS osteosarcoma cells, MCF-7 breast adenocarcinoma and MDA-MB-231 triple negative breast cancer cells. (c) 2021 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2022 Electronic address https://www.sciencedirect.com/science/article/pii/S0300908421001826?via%3Dihub
Number of the records: 1