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Reversible Lectin Binding to Glycan-Functionalized Graphene
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SYSNO ASEP 0551978 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Reversible Lectin Binding to Glycan-Functionalized Graphene Author(s) Koukalová, Tereza (MBU-M)
Kovaříček, P. (CZ)
Bojarová, Pavla (MBU-M) ORCID
Guerra, V. L. P. (CZ)
Vrkoslav, V. (CZ)
Navara, L. (CZ)
Jirka, I. (CZ)
Cebecauer, M. (CZ)
Křen, Vladimír (MBU-M) RID, ORCID
Kalbáč, M. (CZ)Number of authors 10 Article number 6661 Source Title International Journal of Molecular Sciences. - : MDPI
Roč. 22, č. 13 (2021)Number of pages 9 s. Language eng - English Country CH - Switzerland Keywords copper ; graphene ; wheat germ agglutinin ; carbohydrate ; 2D materials ; sensor Subject RIV JJ - Other Materials OECD category Nano-materials (production and properties) R&D Projects LTC20072 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000671186500001 EID SCOPUS 85108207108 DOI 10.3390/ijms22136661 Annotation The monolayer character of two-dimensional materials predestines them for application as active layers of sensors. However, their inherent high sensitivity is always accompanied by a low selectivity. Chemical functionalization of two-dimensional materials has emerged as a promising way to overcome the selectivity issues. Here, we demonstrate efficient graphene functionalization with carbohydrate ligands-chitooligomers, which bind proteins of the lectin family with high selectivity. Successful grafting of a chitooligomer library was thoroughly characterized, and glycan binding to wheat germ agglutinin was studied by a series of methods. The results demonstrate that the protein quaternary structure remains intact after binding to the functionalized graphene, and that the lectin can be liberated from the surface by the addition of a binding competitor. The chemoenzymatic assay with a horseradish peroxidase conjugate also confirmed the intact catalytic properties of the enzyme. The present approach thus paves the way towards graphene-based sensors for carbohydrate-lectin binding. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2022 Electronic address https://www.mdpi.com/1422-0067/22/13/6661
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