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Use of waste substrates for the lipid production by yeasts of the genus Metschnikowia-Screening study
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SYSNO ASEP 0549263 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Use of waste substrates for the lipid production by yeasts of the genus Metschnikowia-Screening study Author(s) Němcová, A. (CZ)
Szotkowski, M. (CZ)
Samek, Ota (UPT-D) RID, ORCID, SAI
Cagáňová, L. (CZ)
Sipiczki, M. (HU)
Márová, I. (CZ)Number of authors 6 Article number 2295 Source Title Microorganisms. - : MDPI
Roč. 9, č. 11 (2021)Number of pages 23 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords yeasts ; Metschnikowia ; animal fat ; lipids ; stress factors ; Raman spectroscopy Subject RIV BH - Optics, Masers, Lasers OECD category Atomic, molecular and chemical physics (physics of atoms and molecules including collision, interaction with radiation, magnetic resonances, Mössbauer effect) Method of publishing Open access Institutional support UPT-D - RVO:68081731 UT WOS 000723780000001 EID SCOPUS 85118337433 DOI 10.3390/microorganisms9112295 Annotation Oleogenic yeasts are characterized by the ability to accumulate increased amounts of lipids under certain conditions. These microbial lipids differ in their fatty acid composition, which allows them to be widely used in the biotechnology industry. The interest of biotechnologists is closely linked to the rising prices of fossil fuels in recent years. Their negative environmental impact is caused by significantly increased demand for biodiesel. The composition of microbial lipids is very similar to vegetable oils, which provides great potential for use in the production of biodiesel. In addition, some oleogenic microorganisms are capable of producing lipids with a high proportion of unsaturated fatty acids. The presented paper´s main aim was to study the production of lipids and lipid substances by yeasts of the genus Metschnikowia, to cultivate crude waste animal fat to study its utilization by yeasts, and to apply the idea of circular economy in the biotechnology of Metschnikowia yeasts. The work focuses on the influence of various stress factors in the cultivation process, such as reduced temperature or nutritional stress through the use of various waste substrates, together with manipulating the ratio of carbon and nitrogen sources in the medium. Yeast production properties were monitored by several instrumental techniques, including gas chromatography and Raman spectroscopy. The amount of lipids and in particular the fatty acid composition varied depending on the strains studied and the culture conditions used. The ability of yeast to produce significant amounts of unsaturated fatty acids was also demonstrated in the work. The most suitable substrate for lipid production was a medium containing glycerol, where the amount of accumulated lipids in the yeast M. pulcherrima 1232 was up to 36%. In our work, the crude animal fat was used for the production of high-value lipids, which to the best of our knowledge is a new result. Moreover, quantitative screening of lipase enzyme activity cultivated on animal fat substrate on selected yeasts of the genus Metschnikowia was performed. We found that for the yeast utilizing glycerol, animal fat seems to be an excellent source of carbon. Therefore, the yeast conversion of crude processed animal fat to value-added products is a valuable process for the biotechnology and food industry. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2022 Electronic address https://www.mdpi.com/2076-2607/9/11/2295
Number of the records: 1