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G-Quadruplex in Gene Encoding Large Subunit of Plant RNA Polymerase II: A Billion-Year-Old Story
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SYSNO ASEP 0544245 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title G-Quadruplex in Gene Encoding Large Subunit of Plant RNA Polymerase II: A Billion-Year-Old Story Author(s) Volna, A. (CZ)
Bartas, M. (CZ)
Karlický, Václav (UEK-B) ORCID, RID, SAI
Nezval, J. (CZ)
Kundratova, K. (CZ)
Pečinka, P. (CZ)
Špunda, Vladimír (UEK-B) ORCID, SAI, RID
Červen, J. (CZ)Number of authors 8 Article number 7381 Source Title International Journal of Molecular Sciences. - : MDPI
Roč. 22, č. 14 (2021)Number of pages 13 s. Language eng - English Country CH - Switzerland Keywords dna-repair ; transcription ; visualization ; instability ; sequences ; molecule ; promoter ; reveal ; system ; server ; evolution ; plant science ; nucleic acids ; circular dichroism ; UV light Subject RIV BO - Biophysics OECD category Biophysics R&D Projects GA18-23702S GA ČR - Czech Science Foundation (CSF) EF16_019/0000797 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Research Infrastructure CzeCOS III - 90123 - Ústav výzkumu globální změny AV ČR, v. v. i. Method of publishing Open access Institutional support UEK-B - RVO:86652079 UT WOS 000676526300001 EID SCOPUS 85109601401 DOI 10.3390/ijms22147381 Annotation G-quadruplexes have long been perceived as rare and physiologically unimportant nucleic acid structures. However, several studies have revealed their importance in molecular processes, suggesting their possible role in replication and gene expression regulation. Pathways involving G-quadruplexes are intensively studied, especially in the context of human diseases, while their involvement in gene expression regulation in plants remains largely unexplored. Here, we conducted a bioinformatic study and performed a complex circular dichroism measurement to identify a stable G-quadruplex in the gene RPB1, coding for the RNA polymerase II large subunit. We found that this G-quadruplex-forming locus is highly evolutionarily conserved amongst plants sensu lato (Archaeplastida) that share a common ancestor more than one billion years old. Finally, we discussed a new hypothesis regarding G-quadruplexes interacting with UV light in plants to potentially form an additional layer of the regulatory network. Workplace Global Change Research Institute Contact Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Year of Publishing 2022 Electronic address https://www.mdpi.com/1422-0067/22/14/7381
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