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Nanoscopic Structural Fluctuations of Disassembling Microtubules Revealed by Label-Free Super-Resolution Microscopy
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SYSNO ASEP 0543280 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Nanoscopic Structural Fluctuations of Disassembling Microtubules Revealed by Label-Free Super-Resolution Microscopy Author(s) Vala, Milan (URE-Y) RID
Bujak, Lukasz (URE-Y)
Marín, Antonio García (URE-Y)
Holanová, Kristýna (URE-Y)
Henrichs, Verena (BTO-N) ORCID
Braun, Marcus (BTO-N) ORCID
Lánský, Zdeněk (BTO-N)
Piliarik, Marek (URE-Y)Number of authors 8 Article number 2000985 Source Title Small Methods. - : Wiley - ISSN 2366-9608
Roč. 5, č. 4 (2021)Number of pages 11 s. Publication form Print - P Language eng - English Country DE - Germany Keywords super-resolution microscopy ; microtubules ; dynamic instability ; iSCAT Subject RIV BH - Optics, Masers, Lasers OECD category Optics (including laser optics and quantum optics) Subject RIV - cooperation Institute of Biotechnology - Physical ; Theoretical Chemistry R&D Projects ED2.1.00/19.0390 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support URE-Y - RVO:67985882 ; BTO-N - RVO:86652036 UT WOS 000611144400001 DOI 10.1002/smtd.202000985 Annotation Microtubules are cytoskeletal polymers of tubulin dimers assembled into protofilaments that constitute nanotubes undergoing periods of assembly and disassembly. Static electron micrographs suggest a structural transition of straight protofilaments into curved ones occurring at the tips of disassembling microtubules. However, these structural transitions have never been observed and the process of microtubule disassembly thus remains unclear. Here, label-free optical microscopy capable of selective imaging of the transient structural changes of protofilaments at the tip of a disassembling microtubule is introduced. Upon induced disassembly, the transition of ordered protofilaments into a disordered conformation is resolved at the tip of the microtubule. Imaging the unbinding of individual tubulin oligomers from the microtubule tip reveals transient pauses and relapses in the disassembly, concurrent with increased organization of protofilament segments at the microtubule tip. These findings show that microtubule disassembly is a discrete process and suggest a stochastic mechanism of switching from the disassembly to the assembly phas Workplace Institute of Radio Engineering and Electronics Contact Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Year of Publishing 2022 Electronic address https://doi.org/10.1002/smtd.202000985
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